International Journal of Anatomical Sciences 2013, 4(1): 09-11

Research Article

Phenytoin Induced Up Regulation of DEFB1 Gene in Albino Rat Testis Gene Microarray Analysis

Rajkumar R, Vatsala Venkatesan, Sriram Thanigai.

Department of Anatomy Mahatma Gandhi Postgraduate Institute of Dental Sciences, Puducherry – 605 006, India.

Department of Anatomy, Sri Balaji Medical College &Hospitals, Chennai – 600 044, Tamil Nadu, India.

Department of Orthopedics, SRM Medical college and Research Centre, Kattankulathur – 603 203, Tamil Nadu, India

Key words : Micro array analyses – gene expression – LPL gene – Phenytoin effects.

Abstract: Phenytoin is widely used against all types of partial and tonic-clonic seizures. phenytoin may alter the release and action of different hormones which may contribute to sexual dysfunction. The present study is aimed at the effect of phenytoin induced differential regulation of DEFB1 gene in albino rat testis .The Albino rat were divided into two groups, control and test, The test group were given 150mgs/kg of phenytoin orally and equal amount of normal saline was given for control group. After 45 days with the rat under deep anesthesia, the testis were removed from the scrotum and stored in liquid nitrogen. The stored specimens of testis of control and tests group were subjected for cDNA microarray analysis. This study showed the differential expression of gene DEFB1 compared to the control group.